Human CD25/IL2RA ELISA Kit
Sensitivity
7.4 pg/mL
Range
31.25-2000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00404 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human CD25/IL2RA ELISA kit is to be used to detect and quantify protein levels of endogenous human CD25/IL2RA. The assay recognizes human CD25/IL2RA. An antibody specific for human CD25/IL2RA has been pre-coated onto the microwells. The human CD25/IL2RA protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human CD25/IL2RA is added to detect the captured human CD25/IL2RA protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human CD25/IL2RA ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 7.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3559 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 92% | 88%-99% |
| Cell culture supernatant | 110% | 104%-115% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 919.6 | 58.1 | 6.3 |
| 2 | 20 | 222.2 | 12.8 | 5.8 |
| 3 | 20 | 66.5 | 4.7 | 7.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 976.4 | 96.4 | 9.9 |
| 2 | 24 | 222.2 | 16.5 | 7.4 |
| 3 | 24 | 68.0 | 5.8 | 8.6 |
Background Information
CD25, also known as the α chain of the IL-2 receptor (IL-2Rα), is a key regulator of T cell function. It is expressed in activated T cells and has been implicated in regulating various immune and autoimmune responses. Its deficiency has been associated with autoimmune phenomena including autoimmune enteropathy and bullous pemphigoid. The soluble CD25 can be found in the serum and may have implications for human disease in both cancer and chronic infection.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms |