CD28 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: Human

Detection: Human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 0.156-10 ng/mL

Cat No : MP00136-1

Print datasheet

Synonyms

CD28, CD28 molecule, Tp44



Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP00136-1 targets CD28 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 0.156-10 ng/mL (Sandwich ELISA);

Capture antibody: CD28 Recombinant antibody, PBS Only (Capture) 83032-1-PBS (230276C11). 100 μg. Concentration 1 mgl/ml.

Detection antibody: CD28 Recombinant antibody, PBS Only (Detector) 83032-2-PBS (230276G6). 100 μg. Concentration 1 mgl/ml.

Alternative CD28 matched antibody pairs: MP00136-2, MP00136-3, MP00136-4

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 83032-1-PBS
Tested Reactivity Human
Immunogen CD28 fusion protein Ag15942
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=7.10 x 10-10M
KOff=1.85 x 10-4M
KOn=2.61 x 105M
Full Name CD28 molecule
Synonyms CD28, CD28 molecule, Tp44
GenBank accession numberBC093698
Gene symbol CD28
UniprotP10747
Gene ID (NCBI) 940
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 83032-2-PBS
Tested Reactivity Human
Immunogen CD28 fusion protein Ag15942
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Affinity
KD=4.51 x 10-10M
KOff=1.00 x 10-4M
KOn=2.22 x 105M
Full Name CD28 molecule
Synonyms CD28, CD28 molecule, Tp44
GenBank accession numberBC093698
Gene symbol CD28
UniprotP10747
Gene ID (NCBI) 940
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.