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Recombinant Human CD28 protein (hFc Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

hFc Tag

生物活性

EC50: 6-24 ng/mL

Cat no : Eg0969

Print datasheet

Synonyms

CD28, CD28 molecule, T-cell-specific surface glycoprotein CD28, Tp44

验证数据展示

  • Purity of Recombinant Human CD28 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD28 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human CD80 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human CD28 (hFc tag) with a linear range of 6-24 ng/mL.

    Immobilized Human CD80 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human CD28 (hFc tag) with a linear range of 6-24 ng/mL.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 
Immobilized Human CD80 (Myc tag, His tag) at 2 μg/mL (100 μL/well) can bind Human CD28 (hFc tag) with a linear range of 6-24 ng/mL.
来源 HEK293-derived Human CD28 protein Asn19-Pro152 (Accession# P10747-1) with a human IgG1 Fc tag at the C-terminus.
基因ID 940
蛋白编号 P10747-1
预测分子量 41.1 kDa
SDS-PAGE 52-65 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Cluster of Differentiation 28 (CD28, also known as Tp44) is one of the proteins constitutively expressed on the surface of 80% of human T cells. CD28 is a highly glycosylated membrane protein, formed by two homodimer disulfide-linked peptides, which shares homology with the immunoglobulin superfamily. CD28 signals play a key role in many T cell processes including cytoskeletal remodeling, production of cytokines, survival, and differentiation. CD28 interactions with the B7 family of costimulatory ligands are essential for initiating antigen-specific T cell responses, upregulating cytokine expression and promoting T cell expansion and differentiation.

参考文献:

1.Hansen JA. et al. (1980).  Immunogenetics. 10(1-4): 247-260.
2.Aruffo A. et al. (1987). Proc Natl Acad Sci U S A. 84(23): 8573-8577.
3.Jonathan H Esensten. et al. (2016). Immunity. 44(5):973-988.
4.D J Lenschow. et al. (1996). Annu Rev Immunol. 14:233-258.