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Human CD3 Magnetic Beads Kit

Cat No : KMS001

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Validation Data Gallery

  • Following cell separation, cell suspension was stained with FITC-CD45(F10-89-4) and PE-CD3(UCHT1) antibodies. All viable cells are gated in the analysis. Left panel: CD3+CD45+ cells before selection. Middle panel: CD3+CD45+ cells after enrichment. Right panel: CD3+CD45+ cells after depletion. Human CD3 magnetic beads kit is tested using PBMC from three donors.

    Following cell separation, cell suspension was stained with FITC-CD45(F10-89-4) and PE-CD3(UCHT1) antibodies. All viable cells are gated in the analysis. Left panel: CD3+CD45+ cells before selection. Middle panel: CD3+CD45+ cells after enrichment. Right panel: CD3+CD45+ cells after depletion. Human CD3 magnetic beads kit is tested using PBMC from three donors.

产品介绍

CD3 is a multimeric protein associated with T cell receptor (TCR) to form a complex involved in antigen recognition and signal transduction. 45%-70% human peripheral blood mononuclear cells (PBMC) express CD3. Human CD3 Magnetic Beads Kit is used for isolation or depletion of human CD3 T lymphocytes from PBMC, whole blood, or other sample types. Following incubation with biotinylated human CD3 antibody and Streptavidin magnetic beads, the cell sample is placed on a magnet. CD3+ cells remain attached to magnetic beads after separation and can be used for downstream applications, such as in cell expansion. CD3- cells remain in supernatant and can also be used for further applications.



KMS001-10:

  · MS001-10: 100µL 10mg/mL streptavidin magnetic beads

  · MS65133-10: 100µL 0.1mg/mL Biotin-CD3 (clone: OKT3)

KMS001-100:

  · MS001-100: 1mL 10mg/mL streptavidin magnetic beads

  · MS65133-100: 1mL 0.1mg/mL Biotin-CD3 (clone: OKT3)


Storage

2-8°C

Storage buffer

Streptavidin beads: PBS, pH7.4, 0.2% BSA and 0.05% Sodium Azide

Biotin Antibody: PBS, pH7.4, 0.2% BSA and 0.09% Sodium Azide

Reactivity

Human

Recommend usage

10µL Biotin-CD3 antibody and 10µL streptavidin beads for 1*107 cells


Publications

ApplicationTitle

Immunol Med

C5a stimulation induces caspase-1 activation and mature IL-1β production in human peripheral blood mononuclear cells

Authors - Yuya Fujita
View Article