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Recombinant Human CD3 gamma protein (rFc Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

rFc Tag

生物活性

未测试

Cat no : Eg2147

Print datasheet

Synonyms

CD3G, CD3 gamma, CD3-gamma, T cell receptor T3 gamma chain, T3G

验证数据展示

  • Purity of Recombinant Human CD3 gamma was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human CD3 gamma was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human CD3 gamma protein Gln23-Ser116 (Accession# P09693) with a rabbit IgG Fc tag at the C-terminus.
基因ID 917
蛋白编号 P09693
预测分子量 37.0 kDa
SDS-PAGE 40-55 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 gamma/CD3G is a single-pass type I membrane protein which consists of an extracellular domain of 94 amino acids, a transmembrane region, and a cytoplasmic domain of 45 amino acids. In addition to this role of signal transduction in T-cell activation, CD3 gamma plays an essential role in the dynamic regulation of TCR expression at the cell surface.

参考文献:

1. J Dietrich, et al. (1994) EMBO J. 13(9):2156-66.
2. J Dietrich, et al. (1996) J Cell Biol. 132(3):299-310. 
3. E A Padlan, et al. (1997) Curr Biol. 7(1):R17-20. 
4. Clifford S Guy, et al. (2009) Immunol Rev. 232(1):7-21.