Human ICAM-1 ELISA Kit
Sensitivity
3.1 pg/mL
Range
62.5-4000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00325 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human ICAM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous human ICAM-1. The assay recognizes human ICAM-1. An antibody specific for human ICAM-1 has been pre-coated onto the microwells. The human ICAM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human ICAM-1 is added to detect the captured human ICAM-1 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human ICAM-1 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant, Saliva, Urine |
| Assay type | Sandwich |
| Sensitivity | 3.1 pg/mL |
| Range | 62.5-4000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3383 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human plasma | 89% | 80%-96% |
| Cell culture supernatant | 92% | 81%-108% |
| Saliva | 91% | 80%-105% |
| Urine | 87% | 80%-97% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 1,720.1 | 66.2 | 3.8 |
| 2 | 20 | 420.5 | 11.9 | 2.8 |
| 3 | 20 | 103.6 | 5.2 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 1,784.5 | 98.5 | 5.5 |
| 2 | 24 | 442.0 | 16.0 | 3.6 |
| 3 | 24 | 112.5 | 5.8 | 5.2 |
Background Information
Intercellular adhesion molecule 1 (ICAM-1, also known as CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin. It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum. Soluble form of ICAM-1 (sICAM-1) has been measured in various body fluids, with increased levels being found in serum from patients with cardiovascular disease, cancer and autoimmune diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | BB2, CD54, ICAM 1, ICAM1, P3.58 |