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Recombinant Human IGF-I/IGF-1 protein (hFc Tag)

ED50

20-80 ng/mL

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

3479

Accession

P05019-1

Cat No : Eg0206

Print datasheet

Synonyms

IBP1, IGF1, IGF1A, IGF1B, IGFI, IGF-I, MGF

验证数据展示

  • Purity of Recombinant Human IGF-I was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IGF-I was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

  • Immobilized Human IGF-I R (GST tag) at 1 μg/mL (100 μL/well) can bind Human IGF-I (hFc tag) with a linear range of 20-80 ng/mL.

    Immobilized Human IGF-I R (GST tag) at 1 μg/mL (100 μL/well) can bind Human IGF-I (hFc tag) with a linear range of 20-80 ng/mL.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Immobilized Human IGF-I R (GST tag) at 1 μg/mL (100 μL/well) can bind Human IGF-I (hFc tag) with a linear range of 20-80 ng/mL.
Source HEK293-derived Human IGF-I protein Gly49-Ala118 (Accession# P05019-1) with a human IgG1 Fc tag at the N-terminus.
Predicted Molecular Mass 33.7 kDa
SDS-PAGE 34-36 kDa
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • 12 months from the date of receipt, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Insulin-like-growth factor 1 (IGF1), a 70 amino-acid peptide hormone is the principal mediator of biochemical effects of growth hormone (GH). IGF1 is an important growth factor in the regulation of cell proliferation and differentiation. IGF1 is largely synthesized in the liver (75%) and, to a lesser extent, in peripheral tissues. IGF1 have been shown to play an essential role in preventing the formation of fatty liver. IGF1 is a potent mitogen and is inhibited by IGF-binding protein-3 (IGFBP3). High serum IGF1 and low IGFBP3 are associated with increased risk of several carcinomas.

References:

1. Adamo ML. et al. (1993) Adv Exp Med Biol. 343:1-11.
2. Adachi Y. et al. (2019) J Gastroenterol Hepatol. 2019 Jun 3.
3. Anisimov VN. et al. (2019) Crit Rev Oncol Hematol. 87(3):201-23.
4. Sonntag WE. et al. (2012) J Gerontol A Biol Sci Med Sci. 67(6):587-98.