Human IL-1 beta ELISA Kit
Sensitivity
0.2 pg/mL
Range
4.7-300 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00592 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human IL-1 beta ELISA kit is to be used to detect and quantify protein levels of endogenous human IL-1 beta. The assay recognizes human IL-1 beta. An antibody specific for human IL-1 beta has been pre-coated onto the microwells. The human IL-1 beta protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human IL-1 beta is added to detect the captured human IL-1 beta protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-1 beta ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 0.2 pg/mL |
| Range | 4.7-300 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3553 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 94% | 87%-99% |
| Cell culture supernatant | 117% | 104%-127% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 86.9 | 1.9 | 2.2 |
| 2 | 8 | 22.1 | 0.8 | 3.6 |
| 3 | 8 | 11.8 | 0.8 | 6.7 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 142.3 | 3.4 | 2.4 |
| 2 | 16 | 34.7 | 1.5 | 4.4 |
| 3 | 16 | 17.3 | 0.7 | 3.8 |
Background Information
Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1 beta and their naturally occurring inhibitor IL-1RN. Interleukin 1 beta (IL-1 beta), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1 beta is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1 beta is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Catabolin, IL 1, IL 1 beta, IL1 beta, IL1- beta |