Human IL-1R2 ELISA Kit
Sensitivity
1.3 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Human
Cat No : KE00288
Synonyms
CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1R2, IL-1R2, IL1RB, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II
Validation Data Gallery
Product Information
KE00288 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human IL-1R2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1R2. The assay recognizes human IL-1R2. An antibody specific for IL-1R2 has been pre-coated onto the microwells. The IL-1R2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human IL-1R2 is added to detect the captured human IL-1R2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human IL-1R2 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 1.3 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 7850 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 102% | 90%-121% |
| Cell culture supernatant | 107% | 80%-124% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 529.2 | 34.8 | 6.6 |
| 2 | 20 | 105.9 | 3.9 | 3.7 |
| 3 | 20 | 30.1 | 1.9 | 6.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 534.7 | 24.2 | 4.5 |
| 2 | 24 | 121.4 | 5.8 | 4.8 |
| 3 | 24 | 23.1 | 2.2 | 9.5 |
Background Information
Interleukin-1 receptor type 2 (IL-1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. IL-1R2 binds interleukin alpha (IL-1A), interleukin beta (IL-1B), and interleukin 1 receptor, type I (IL-1R1/IL-1RA), and acts as a decoy receptor that inhibits the activity of its ligands. It exists in both a membrane bound and soluble form. Soluble molecules are found in cell culture supernatant and in biological fluids under several physiopathological conditions.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1R2, IL-1R2, IL1RB, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II |