Human IL-1R2 ELISA Kit

Sensitivity

1.3 pg/mL

Range

15.6-1000 pg/mL

Reactivity

Human

Cat No : KE00288

说明书下载

Synonyms

CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1R2, IL-1R2, IL1RB, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II



Product Information

KE00288 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Human IL-1R2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1R2. The assay recognizes human IL-1R2. An antibody specific for IL-1R2 has been pre-coated onto the microwells. The IL-1R2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for human IL-1R2 is added to detect the captured human IL-1R2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human IL-1R2 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatant
Assay type Sandwich
Sensitivity 1.3 pg/mL
Range 15.6-1000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 7850

Recovery

Sample TypeAverageRange
Human serum102%90%-121%
Cell culture supernatant107%80%-124%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120529.234.86.6
220105.93.93.7
32030.11.96.5

InterAssay

Samplenmean ( pg/mL)SDCV%
124534.724.24.5
224121.45.84.8
32423.12.29.5

Background Information

Interleukin-1 receptor type 2 (IL-1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. IL-1R2 binds interleukin alpha (IL-1A), interleukin beta (IL-1B), and interleukin 1 receptor, type I (IL-1R1/IL-1RA), and acts as a decoy receptor that inhibits the activity of its ligands. It exists in both a membrane bound and soluble form. Soluble molecules are found in cell culture supernatant and in biological fluids under several physiopathological conditions.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms CD121b, CDw121b, IL 1R 2, IL 1R beta, IL 1RT 2, IL 1RT2, IL1R2, IL-1R2, IL1RB, Interleukin 1 receptor beta, Interleukin 1 receptor type 2, Interleukin 1 receptor type II