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Recombinant Human IL-12B protein (His Tag)

ED50

/

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

3593

Accession

P29460

Cat No : Eg0431

Print datasheet

Synonyms

IL-12/IL-23 p40,IL12B,CLMF p40,Cytotoxic lymphocyt

验证数据展示

  • Purity of Recombinant Human IL-12B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IL-12B was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Human IL-12B protein Ile23-Ser328 (Accession# P29460) with a His tag at the C-terminus.
Predicted Molecular Mass 38.5 kDa
SDS-PAGE 40-48 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells. IL-12 p40 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Overexpression of IL-12 p40 was observed in the central nervous system of patients with multiple sclerosis (MS), suggesting a role of this cytokine in the pathogenesis of the disease. The promoter polymorphism of IL-12 p40 has been reported to be associated with the severity of atopic and non-atopic asthma in children.

References:

1. Cooper AM. et al. (2007) Trends Immunol. 28:33-8.
2. Wang SZ. et al. (2004) J Immunol. 173:4040-9.
3. Bielekova B. et al. (2012) PLoS One. 7:e48370.
4. Xu J. et al. (2016) Cell Death Differ. 23:1941-1951.