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Recombinant Human IL-22 protein (His Tag)

ED50

/

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

50616

Accession

Q9GZX6

Cat No : Eg0228

Print datasheet

Synonyms

IL22,Cytokine Zcyto18,IL 21,IL 22,IL D110

验证数据展示

  • Purity of Recombinant Human IL-22 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human IL-22 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
Not tested
Source HEK293-derived Human IL-22 protein Met+Ala34-Ile179 (Accession# Q9GZX6) with a His tag at the N-terminus.
Predicted Molecular Mass 17.6 kDa
SDS-PAGE 20-27 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Interleukin 22 (IL-22) is a member of the human type I interferon family, which includes IL10. IL-22 has the potential to interact with IL10 because it binds to the IL-10R2c chain with IL-22R1 in its receptor complex. Binding can be blocked by the soluble receptor, IL-22 binding protein (IL-22BP). Although known as a Th17 cytokine, IL-22 is also expressed by a wide range of immune cells, including NK T, γδ T, and NK cells. IL-22 can contribute to immune disease through the stimulation of inflammatory responses, S100s and defensins. IL-22 also promotes hepatocyte survival in the liver and epithelial cells in the lung and gut similar to IL-10.

References:

1. Pestka S. et al. (2004) Annu Rev Immunol. 22: 929-79.
2. Wolk K. et al. (2002) J Immunol 168(11): 5397-5402.
3. Zheng Y et al. (2007) Nature 445: 648-651.
4. Zheng Y et al. (2008) Nat Med 14: 282-289.
5. Xie MH, et al. (2000) J Biol Chem. 275: 31335-9.
6. Jones BC, et al. (2008) 16:1333-44.