IgG Heavy chain Monoclonal antibody

Human IgG Heavy chain Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1C6B4

Cat no : 67760-1-Ig

Print datasheet

Synonyms



经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67760-1-Ig targets Human IgG Heavy chain in WB, IHC, IF-P, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human
Immunogen nature protein 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Human IgG Heavy chain
Synonyms
Calculated Molecular Weight 155 kDa
Observed Molecular Weight~50 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2918528
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the heavy chain of human IgG.

实验方案

Product Specific Protocols
WB protocol for Human IgG Heavy chain antibody 67760-1-IgDownload protocol
IHC protocol for Human IgG Heavy chain antibody 67760-1-IgDownload protocol
IF protocol for Human IgG Heavy chain antibody 67760-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols