• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

IgG Kappa chain Monoclonal antibody

Human IgG Kappa chain Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

4D11F7

Cat no : 67761-1-Ig

Print datasheet

Synonyms

验证数据展示

  • IHC staining of human tonsillitis using 67761-1-Ig

    IHC staining of human tonsillitis using 67761-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67761-1-Ig

    IHC staining of human tonsillitis using 67761-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67761-1-Ig

    IHC staining of human tonsillitis using 67761-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human plasma using 67761-1-Ig

    WB analysis of human plasma using 67761-1-Ig

    16 nL human plasma were subjected to SDS PAGE followed by western blot with 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67761-1-Ig targets Human IgG Kappa chain in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human
Immunogen nature protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Human IgG Kappa chain
Synonyms
Calculated Molecular Weight 155 kDa
Observed Molecular Weight~25 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2918529
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the Kappa chain of human IgG,

实验方案

Product Specific Protocols
WB protocol for Human IgG Kappa chain antibody 67761-1-IgDownload protocol
IHC protocol for Human IgG Kappa chain antibody 67761-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols