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验证数据展示

  • IHC staining of human tonsillitis using 67761-1-Ig

    IHC staining of human tonsillitis using 67761-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67761-1-Ig

    IHC staining of human tonsillitis using 67761-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 67761-1-Ig

    IHC staining of human tonsillitis using 67761-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of human plasma using 67761-1-Ig

    WB analysis of human plasma using 67761-1-Ig

    16 nL human plasma were subjected to SDS PAGE followed by western blot with 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67761-1-Ig

    IHC staining of human tonsillitis using 67761-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67761-1-Ig (Human IgG Kappa chain antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Human IgG Kappa chain Monoclonal antibody

Human IgG Kappa chain Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67761-1-Ig

产品说明书

CloneNo. 4D11F7

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

IgG Kappa chain, human IgG

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67761-1-Ig targets Human IgG Kappa chain in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 nature protein 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 Human IgG Kappa chain
别名 IgG Kappa chain, human IgG
计算分子量 155 kDa
观测分子量~25 kDa
基因名称
Gene ID (NCBI)
RRIDAB_2918529
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects the Kappa chain of human IgG,

实验方案

Product Specific Protocols
WB protocol for Human IgG Kappa chain antibody 67761-1-IgDownload protocol
IHC protocol for Human IgG Kappa chain antibody 67761-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols