• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of human plasma using 67762-1-Ig

    WB analysis of human plasma using 67762-1-Ig

    80 nL human plasma were subjected to SDS PAGE followed by western blot with 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 67762-1-Ig

    IHC staining of human tonsillitis using 67762-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgG lambda chain antibody included
  • IHC staining of human tonsillitis using 67762-1-Ig

    IHC staining of human tonsillitis using 67762-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67762-1-Ig (Human IgG lambda chain antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Human IgG lambda chain antibody included
  • IF Staining of human appendicitis using 67762-1-Ig

    IF Staining of human appendicitis using 67762-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Human IgG lambda chain antibody (67762-1-Ig, Clone: 1D12B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

Human IgG lambda chain Monoclonal antibody

Human IgG lambda chain Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67762-1-Ig

产品说明书

CloneNo. 1D12B5

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

IgG Lambda Light Chain, IGLC1, Immunoglobulin lambda constant 1, IGLC, 1D12B5

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman appendicitis tissue
Planning an IHC experiment? We recommend our IHCeasy Human IgG lambda chain Ready-To-Use IHC Kit. Human IgG lambda chain primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®594 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67762-1-Ig targets Human IgG lambda chain in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 Human IgG lambda chain fusion protein Ag14783 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 immunoglobulin lambda constant 1 (Mcg marker)
别名 IgG Lambda Light Chain, IGLC1, Immunoglobulin lambda constant 1, IGLC, 1D12B5
计算分子量 233 aa, 25 kDa
观测分子量~25 kDa
GenBank蛋白编号BC007782
基因名称 IgG Lambda Light Chain
Gene ID (NCBI) 3537
RRIDAB_2918530
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

This antibody detects Lambda chain of human IgG.

实验方案

Product Specific Protocols
WB protocol for Human IgG lambda chain antibody 67762-1-IgDownload protocol
IHC protocol for Human IgG lambda chain antibody 67762-1-IgDownload protocol
IF protocol for Human IgG lambda chain antibody 67762-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols