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Recombinant Human MSLN (Myc Tag, His Tag)

ED50

/

Species

Human

Purity

>90 %, SDS-PAGE

GeneID

10232

Accession

Q13421

Cat No : Eg31658

Print datasheet

Synonyms

CAK1, CAK1 antigen, Megakaryocyte-potentiating factor, Mesothelin, Mesothelin, cleaved form, MPF, MPF Mesothelin, cleaved form, MSLN, Pre-pro-megakaryocyte-potentiating factor, SMR

验证数据展示

Technical Specifications

Purity >90 %, SDS-PAGE
Endotoxin Level <1.0 EU/μg protein, LAL method
Biological Activity 
/
Source HEK293-derived Human MSLN protein Glu296-Ser606 (Accession# Q13421) with a Myc Tag and a His Tag at the C-terminus.
Predicted Molecular Mass 37.1 kDa
SDS-PAGE 40-70 kDa, reducing (R) conditions
Formulation Lyophilized from sterile PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
Reconstitution Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
Storage
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

Background

Mesothelin (MSLN) is a glycosylphosphatidylinositol-linked membrane glycoprotein which is highly expressed in a variety of tumors and is also expressed in mesothelial cells of healthy individuals, but at low levels. Therefore, it can be considered as a promising target protein for tumor-targeted therapy. In the tumor environment, MSLN plays an important role in survival, proliferation, and migration/invasion of cancer cells as well as in drug resistance.

References:

1. Zeng W, Pan J, Fang Z, Jia J, Zhang R, He M, Zhong H, He J, Yang X, Shi Y, Zhong B, Zeng J, Fu B, Huang M, Liu H. A Novel PD-L1-Containing MSLN Targeting Vaccine for Lung Cancer Immunotherapy. Front Immunol. 2022 Jun 20;13:925217.
2. Schoutrop E, El-Serafi I, Poiret T, Zhao Y, Gultekin O, He R, et al.. Mesothelin-Specific CAR T Cells Target Ovarian Cancer. Cancer Res (2021) 81:3022–35.
3. Weidemann S, Gagelmann P, Gorbokon N, Lennartz M, Menz A, Luebke AM, et al.. Mesothelin Expression in Human Tumors: A Tissue Microarray Study on 12,679 Tumors. Biomedicines (2021) 9:397.
4. Le K, Wang J, Zhang T, Guo Y, Chang H, Wang S, et al.. Overexpression of Mesothelin in Pancreatic Ductal Adenocarcinoma (PDAC). Int J Med Sci (2020) 17:422–7.
5. Del Bano J, Florès-Florès R, Josselin E, Goubard A, Ganier L, Castellano R, et al.. A Bispecific Antibody-Based Approach for Targeting Mesothelin in Triple Negative Breast Cancer. Front Immunol (2019) 10:1593.