Human NGAL ELISA Kit
Sensitivity
3.2 pg/mL
Range
19.5-1250 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00327 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The NGAL ELISA kit is to be used to detect and quantify protein levels of endogenous NGAL. The assay recognizes human NGAL. An antibody specific for NGAL has been pre-coated onto the microwells. The NGAL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-NGAL protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human NGAL ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Human plasma,Cell culture supernatants,Urine,Saliva |
| Assay type | Sandwich |
| Sensitivity | 3.2 pg/mL |
| Range | 19.5-1250 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 3934 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Cell culture supernatants | 92% | 81%-106% |
| Urine | 91% | 78%-105% |
| Saliva | 98% | 87%-118% |
| Human plasma | 93% | 80%-103% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 542.8 | 25.0 | 4.6 |
| 2 | 20 | 125.5 | 8.6 | 6.8 |
| 3 | 20 | 30.3 | 2.3 | 7.5 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 572.6 | 37.1 | 6.5 |
| 2 | 24 | 135.0 | 10.2 | 7.5 |
| 3 | 24 | 32.1 | 2.8 | 8.8 |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL) also known as Lipocalin-2 (LCN2), is a secreted glycoprotein that belongs to the lipocalin protein family. Several roles of NGAL was identified, including iron trafficking, chemotactic, and promote cell differentiation and proliferation. NGAL was originally found in human neutrophils, and expressed in tissues containing renal, uterus, liver, spleen, as well as cardiomyocytes, neurons and immune cells. NGAL is a significant biomarker of cardiovascular diseases, ischemic stroke, acute renal injury, inflammation and metabolic diseases, and it can be detected in human serum, saliva and urine.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | 24p3, HNL, LCN2, lipocalin 2, NGAL, Oncogene 24p3, p25 |