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Recombinant Human PGF/PIGF protein (His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg0653

Print datasheet

Synonyms

PGF, PGFL, PIGF, PLGF, PGF/PIGF

验证数据展示

  • Purity of Recombinant Human PGF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

    Purity of Recombinant Human PGF was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human PGF/PIGF protein Ala21-Arg149 (Accession# P49763-2) with a His tag at the C-terminus.
基因ID 5228
蛋白编号 P49763-2
预测分子量 15.6 kDa
SDS-PAGE 18-25 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

PlGF (placenta growth factor), also named as PGF, is an angiogenic factor which belongs to vascular endothelial growth factor (VEGF) family. The human PlGF gene is located on chromosome 14q14 and encodes 4 isoforms of PlGF. The protein is secreted as a glycosylated homodimer and PlGF-1 and -3 are diffusible isoforms whereas PlGF-2 and PlGF-4 have heparin binding domains. PlGF was originally identified in the placenta, where it regulates growth and differentiation of trophoblasts. It is also expressed in umbilical vein endothelial cells and other non-placental tissues, like the thyroid gland and developing lung tissue. PlGF has angiogenic properties, enhancing survival, growth and migration of endothelial cells in vitro, and promotes vessel formation in certain in-vivo models. Serum levels of PIGF and sFlt-1 (also known as soluble VEGF receptor-1) are altered in women with preeclampsia. PIGF is a potential biomarker for preeclampsia, a condition in which blood vessels in the placenta are too narrow, resulting in high blood pressure.

参考文献:

1.KhalilA.etal.(2008)PLoSOne.23;3(7):e2766.
2.DewerchinM.etal.(2012)ColdSpringHarbPerspectMed.1;2(8).
3.OuraH.et.at.(2003)Blood.101(2):560-7.
4.DuhigKE.etal.(2019)Lancet.393(10183):1807-1818.