Human Prolactin ELISA Kit
Validation Data Gallery
Product Information
KE00172 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The PRL ELISA kit is to be used to detect and quantify protein levels of endogenous PRL. The assay recognizes human PRL. An antibody specific for PRL has been pre-coated onto the microwells. The PRL protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for PRL is added to detect the captured PRL protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Prolactin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatants, Human milk 备注：对未验证样本，我们提供技术支持和售后保障。 |
| Assay type | Sandwich |
| Sensitivity | 1.4 pg/mL |
| Range | 31.25-2000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 5617 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| human serum | 99% | 89%-110% |
| Cell culture supernatants | 94% | 78%-109% |
| human milk | 103% | 89%-123% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 101.7 | 8.5 | 8.4 |
| 2 | 20 | 305.6 | 5.6 | 1.8 |
| 3 | 20 | 1,054.2 | 21.8 | 2.1 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 72.9 | 4.1 | 5.1 |
| 2 | 24 | 264.1 | 7.1 | 2.7 |
| 3 | 24 | 958.0 | 28.2 | 2.9 |
Background Information
Prolactin (PRL) is a pleiotropic hormone which is mainly synthetized by the lactotrophs of the anterior pituitary gland. Prolactin has a wide variety of effects. It causes the breasts to grow and make milk during pregnancy and after birth. Prolactin levels are normally high for pregnant women and new mothers. Prolactin also plays an essential role in metabolism, regulation of the immune system and pancreatic development. Normally during pregnancy prolactin serum levels are increased to hold of insulin resistance (IR). As well, prolactin is involved in regulation of glucose metabolism, since high prolactin levels are associated with low incidence of type 2 diabetes mellitus (T2DM).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | PRL, prolactin |
FAQ
1、我的样本稀释液有限，如何处理稀释倍数较大的样本？
我们建议您对样本进行梯度稀释。例如，人血清样本需要1000000倍稀释时，您可以分3个梯度，每个梯度100倍，进行稀释：
第一步：取2 uL人血清样本，加入198 uL样本稀释液，混匀得到100倍稀释的人血清样本
第二步：取2uL 100倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到10000倍稀释的人血清样本
第三步：取2uL 10000倍稀释的人血清样本+ 198 uL样本稀释液进行稀释，混匀得到1000000倍稀释的人血清样本。
如果样本需要进一步稀释，您可以使用更多梯度。除了我们推荐的样本稀释液，不建议您使用其他溶液或者水稀释。如果您的样本稀释液仍然不足，请联系我们的客服为您提供更多样本稀释液。
Publications
| Species | Sample Type | Title |
|---|---|---|
Reprod Sci A Novel Method to Repair Thin Endometrium and Restore Fertility Based on Menstruation-Derived Stem Cell | ||
Jpn J Nurs Sci A program for accelerating lactogenesis II using a breast pump shortly after cesarean section in Japan: A feasibility mixed-methods study. |
