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Recombinant Human SIRP alpha/CD172a protein (His Tag)

种属

Human

纯度

>90 %, SDS-PAGE

标签

His Tag

生物活性

未测试

Cat no : Eg0625

Print datasheet

Synonyms

SIRP Alpha, SIRP Alpha/CD172a, SIRPA, BIT, Brain Ig-like molecule with tyrosine-based activation motifs

验证数据展示

  • Purity of Recombinant Human SIRP alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human SIRP alpha was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Not tested
来源 HEK293-derived Human SIRP alpha protein Glu31-Arg370 (Accession# P78324-1) with a His tag at the C-terminus.
基因ID 140885
蛋白编号 P78324-1
预测分子量 38.1 kDa
SDS-PAGE 50-70 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

SIRP Alpha, also known as CD172a or SHPS-1, is a transmembrane glycoprotein that belongs to the SIRP family. SIRP Alpha is a receptor with immunoreceptor tyrosine-based inhibition motifs in its cytoplasmic domain expressed by myeloid cells, including monocytes, macrophages, granulocytes, and a subset of dendritic cells. After activation of SIRP Alpha, Src homology 2 domain-containing protein tyrosine phosphatase-1 (SHP-1) is recruited and regulates receptor tyrosine kinase coupled signaling, participating in the regulation of phagocytosis and polarization of macrophages. SIRP Alpha binds to CD47, a receptor present on all cells (and frequently overexpressed in cancer cells), and this interaction provides a ‘do-not-eat-me’ signal to prevent phagocytosis.

参考文献:

1. Sarfati M, et al. (2008) Curr Drug Targets. 9(10):842-50.
2. Barclay AN, et al. (2014) Annu Rev Immunol. 32:25-50.
3. de Vos AF, et al. (2020) Nat Immunol. 21(6):601-603.
4. Shen Q, et al. (2022) Front Immunol. 13:865579.