Human Synaptophysin ELISA Kit
Sensitivity
0.01 ng/mL
Range
0.313-20 ng/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00381 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human Synaptophysin ELISA kit is to be used to detect and quantify protein levels of endogenous human Synaptophysin. The assay recognizes human Synaptophysin. An antibody specific for human Synaptophysin has been pre-coated onto the microwells. The human Synaptophysin protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for human Synaptophysin is added to detect the captured human Synaptophysin protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human Synaptophysin ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell lysate |
| Assay type | Sandwich |
| Sensitivity | 0.01 ng/mL |
| Range | 0.313-20 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 6855 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Serum | 104% | 91%-111% |
| Cell lysate | 93% | 85%-107% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 8.44 | 0.22 | 2.65 |
| 2 | 20 | 1.98 | 0.06 | 2.90 |
| 3 | 20 | 0.49 | 0.02 | 4.35 |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 8.85 | 0.44 | 5.00 |
| 2 | 24 | 2.06 | 0.10 | 4.77 |
| 3 | 24 | 0.55 | 0.03 | 4.88 |
Background Information
Synaptophysin (SYP, also known as major synaptic vesicle protein p38) is a 38-kDa integral membrane glycoprotein that regulates synaptic vesicle endocytosis. It is the most abundant synaptic vesicle protein by mass. Synaptophysin is present in neuroendocrine cells and neurons that participate in synaptic transmission. Synaptic proteins including synaptophysin are reduced in brain tissue from patients with Alzheimer's disease. Synaptophysin is a useful marker for the identification of neuroendocrine cells and neoplasms.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Synaptophysin,SYP,MRX96,SYN |