Human TNFSF15 ELISA Kit
Sensitivity
14.4 pg/mL
Range
78.1-5000 pg/mL
Reactivity
Human
Validation Data Gallery
Product Information
KE00296 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The human TNFSF15 ELISA kit is to be used to detect and quantify protein levels of endogenous TNFSF15. The assay recognizes human TNFSF15. An antibody specific for TNFSF15 has been pre-coated onto the microwells. The TNFSF15 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another horseradish peroxidase (HRP)-conjugated antibody specific for TNFSF15 is added to detect the captured TNFSF15 protein. For signal development, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.
| Product name | Human TNFSF15 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Cell culture supernatant |
| Assay type | Sandwich |
| Sensitivity | 14.4 pg/mL |
| Range | 78.1-5000 pg/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 9966 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Human serum | 98% | 89%-114% |
| Cell culture supernatant | 85% | 71%-92% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | 2,261.0 | 155.2 | 6.9 |
| 2 | 20 | 518.4 | 25.6 | 4.9 |
| 3 | 20 | 130.3 | 6.5 | 5.0 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 24 | 2,313.6 | 211.5 | 9.1 |
| 2 | 24 | 521.6 | 48.3 | 9.3 |
| 3 | 24 | 129.3 | 12.0 | 9.3 |
Background Information
Tumor necrosis factor ligand superfamily member 15 (TNFSF15) is also named as TL1 and VEGI. And it belongs to the tumor necrosis factor family. It is a receptor for TNFRSF25 and TNFRSF6B. VEGI induced degradation of IκB alpha, and nuclear translocation of p65 subunit of NFκB by activating NFκB. TL1A mediates activation and apoptosis of NFκB in DR3-expressing cell lines. Overexpression by cancer cells of a secretable VEGI fusion protein resulted in abrogation of xenograft tumor progression. The isoforms show endothelial cell-specific expression and are generated from a 17 kb human gene by alternative splicing. In addition, VEGI can inhibit vascular endothelial growth and angiogenesis (in vitro).
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | TL1, TL1A, TNF ligand related molecule 1, TNFSF15, VEGI, VEGI192A |