• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Recombinant Human gp130/IL6ST protein (His Tag)

种属

Human

纯度

>95 %, SDS-PAGE

标签

His Tag

生物活性

EC50: 90-370 ng/mL

Cat no : Eg0654

Print datasheet

Synonyms

gp130/IL6ST, IL6ST, CD130, Glycoprotein 130, GP130

验证数据展示

  • Purity of Recombinant Human gp130 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

    Purity of Recombinant Human gp130 was determined by SDS-PAGE. The protein was resolved in an SDS-PAGE in reducing (R) and non-reducing (NR) conditions and stained using Coomassie blue.

  • Immobilized Human gp130 (His tag) at 1 μg/mL (100 μL/well) can bind Human IL-6R alpha (Myc tag, His tag) with a linear range of 90-370 ng/mL.

    Immobilized Human gp130 (His tag) at 1 μg/mL (100 μL/well) can bind Human IL-6R alpha (Myc tag, His tag) with a linear range of 90-370 ng/mL.

产品信息

纯度 >95 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性 Immobilized Human gp130 (His tag) at 1 μg/mL (100 μL/well) can bind Human IL-6R alpha (Myc tag, His tag) with a linear range of 90-370 ng/mL.
来源 HEK293-derived Human gp130 protein Glu23-Ile618 (Accession# P40189-1) with a His tag at the C-terminus.
基因ID 3572
蛋白编号 P40189-1
预测分子量 68.5 kDa
SDS-PAGE 80-100 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Glycoprotein 130 (gp130, also known as IL6ST, CD130 or IL6-beta) is a ubiquitously expressed, signal-transducing receptor that serves as the signal transduction unit for IL-6 family of cytokines, including IL-6, IL-11, IL-27, leukemia inhibitory factor (LIF), OSM, ciliary neurotrophic factor (CNTF), cardiotrophin 1 (CT-1), and cardiotrophin-like cytokine (CLC). These cytokines signal through the gp130/Jak/STAT pathway. Binding of IL-6 to IL-6R induces gp130 homodimerization and formation of a high-affinity receptor complex, which activates Jaks. That causes phosphorylation of gp130 tyrosine residues which in turn activates STAT3. gp130 is a type I transmembrane protein, and can also exist as a soluble form (sgp130). sgp130 binds to sIL-6R/IL-6 complexes and prevents their interactions with membrane-anchored gp130 on target cells.

参考文献:

1. Taga T, Kishimoto T. (1997).  Annu Rev Immunol. 15:797-819.
2. Montero-Julian FA, et al. (1997). Clin Cancer Res. 3(8):1443-1451.
3. Heinrich PC, et al. (1998). Biochem J. 334 (Pt 2)(Pt 2):297-314.
4. Silver JS, Hunter CA. (2010). J Leukoc Biol. 88(6):1145-1156.