验证数据展示
经过测试的应用
Positive IHC detected in | rat brain tissue, mouse brain tissue, mouse spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | rat brain tissue |
推荐稀释比
应用 | 推荐稀释比 |
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Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30523-1-AP targets IBA1 in IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.
经测试应用 | IHC, IF-P, ELISA Application Description |
经测试反应性 | mouse, rat |
免疫原 | IBA1 fusion protein Ag30575 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | allograft inflammatory factor 1 |
别名 | AI607846, AIF 1, Aif1, D17H6S50E, G1, Iba1 |
计算分子量 | 17 kDa |
GenBank蛋白编号 | NM_019467 |
基因名称 | IBA1 |
Gene ID (NCBI) | 11629 |
RRID | AB_3086348 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O70200 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.
实验方案
Product Specific Protocols | |
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IHC protocol for IBA1 antibody 30523-1-AP | Download protocol |
IF protocol for IBA1 antibody 30523-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |