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验证数据展示

  • IHC staining of rat brain using 30523-1-AP

    IHC staining of rat brain using 30523-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30523-1-AP (IBA1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of rat brain using 30523-1-AP

    IHC staining of rat brain using 30523-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 30523-1-AP (IBA1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat brain using 30523-1-AP

    IF Staining of rat brain using 30523-1-AP

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (30523-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

IBA1 Polyclonal antibody

IBA1 Polyclonal Antibody for IHC, IF-P, ELISA
Cat No. 30523-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

IHC, IF-P, ELISA

Synonyms

AI607846, AIF 1, Aif1, D17H6S50E, G1, Iba1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive IHC detected inrat brain tissue, mouse brain tissue, mouse spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30523-1-AP targets IBA1 in IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications IHC, IF-P, ELISA Application Description
Tested Reactivity mouse, rat
Immunogen IBA1 fusion protein Ag30575 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name allograft inflammatory factor 1
Synonyms AI607846, AIF 1, Aif1, D17H6S50E, G1, Iba1
Calculated Molecular Weight 17 kDa
GenBank Accession NumberNM_019467
Gene Symbol IBA1
Gene ID (NCBI) 11629
RRIDAB_3086348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO70200
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.

实验方案

Product Specific Protocols
IHC protocol for IBA1 antibody 30523-1-APDownload protocol
IF protocol for IBA1 antibody 30523-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols