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IBA1 Recombinant antibody, PBS Only

IBA1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4D5

Cat no : 81728-1-PBS

Print datasheet

Synonyms

AIF1, G1, allograft inflammatory factor 1, AIF-1, AIF 1

验证数据展示

  • IF Staining of adult mouse cerebellum tissue using 81728-1-RR (same clone as 81728-1-PBS)

    IF Staining of adult mouse cerebellum tissue using 81728-1-RR (same clone as 81728-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using IBA1 antibody (81728-1-RR) at dilution of 1:500 and Alexa Fluor 594 AffiniPure Donkey Anti-Rabbit IgG (H+L). Heat mediated antigen retrieval with PBS buffer (pH 7.4). (Image produced by Watt Lab at McGill University) This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • WB analysis of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

    WB analysis of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IBA1 antibody included
  • IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    IHC staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 81728-1-RR (IBA1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IBA1 antibody included
  • IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • WB analysis using 81728-1-RR (same clone as 81728-1-PBS)

    WB analysis using 81728-1-RR (same clone as 81728-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81728-1-RR (IBA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    IF Staining of rat brain using 81728-1-RR (same clone as 81728-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed rat brain tissue using IBA1 antibody (81728-1-RR, Clone: 4D5 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

  • FC experiment of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

    FC experiment of THP-1 using 81728-1-RR (same clone as 81728-1-PBS)

    1x10^6 THP-1 cells were intracellularly stained with 0.4 ug IBA1 Recombinant antibody (81728-1-RR, Clone:4D5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 81728-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy IBA1 Ready-To-Use IHC Kit. IBA1 primary antibody included.

产品信息

81728-1-PBS targets IBA1 in WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IF-Fro, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IBA1 fusion protein Ag1363 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name allograft inflammatory factor 1
Synonyms AIF1, G1, allograft inflammatory factor 1, AIF-1, AIF 1
Calculated Molecular Weight 17 kDa
Observed Molecular Weight17 kDa
GenBank Accession NumberBC009474
Gene Symbol IBA1
Gene ID (NCBI) 199
ENSEMBL Gene IDENSG00000204472
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55008
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IBA1 is a 143 amino acid cytoplasmic, inflammation response scaffold protein. It is constitutively expressed in monocytes and macrophages and is known to be involved in macrophage activation. It is a marker of activated macrophage. Expression of IBA1 is up-regulated in activated microglia following facial nerve axotomy, ischemia, and several brain diseases, thereby implicating it in the activated phenotypes of microglia.