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Mouse ICAM-1/CD54 Recombinant antibody, PBS Only

ICAM-1/CD54 Uni-rAbTM Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E23

Cat no : 82827-1-PBS

Print datasheet

Synonyms

CD54, ICAM-1, MALA-2, Icam1, ICAM 1

验证数据展示

  • WB analysis using 82827-1-RR (same clone as 82827-1-PBS)

    WB analysis using 82827-1-RR (same clone as 82827-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82827-1-RR (ICAM-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 82827-1-RR (same clone as 82827-1-PBS)

    IHC staining of mouse lung using 82827-1-RR (same clone as 82827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 82827-1-RR (same clone as 82827-1-PBS)

    IHC staining of mouse lung using 82827-1-RR (same clone as 82827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 82827-1-RR (ICAM-1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82827-1-PBS in a different storage buffer formulation.

产品信息

82827-1-PBS targets ICAM-1/CD54 in WB, IHC, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intercellular adhesion molecule 1
Synonyms CD54, ICAM-1, MALA-2, Icam1, ICAM 1
Calculated Molecular Weight59 kDa
Observed Molecular Weight85-110 kDa
GenBank Accession NumberNM_010493
Gene Symbol ICAM-1
Gene ID (NCBI) 15894
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP13597
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784).