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ICAM2 Polyclonal antibody

ICAM2 Polyclonal Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27322-1-AP

Print datasheet

Synonyms

CD102, ICAM 2, ICAM2

验证数据展示

  • WB analysis using 27322-1-AP

    WB analysis using 27322-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27322-1-AP (ICAM2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • FC experiment of HUVEC using 27322-1-AP

    FC experiment of HUVEC using 27322-1-AP

    1X10^6 HUVEC cells were stained with 0.2 ug Anti-Human ICAM2 (27322-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

经过测试的应用

Positive WB detected inHL-60 cells, mouse lung tissue, Jurkat cells, HUVEC cells
Positive FC detected inHUVEC cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27322-1-AP targets ICAM2 in WB, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications FC, WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen ICAM2 fusion protein Ag25952 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intercellular adhesion molecule 2
Synonyms CD102, ICAM 2, ICAM2
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 55-80 kDa
GenBank Accession NumberBC003097
Gene Symbol ICAM2
Gene ID (NCBI) 3384
RRIDAB_2880843
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13598
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ICAM2 is a cell adhesion protein having important roles in cell migration, especially during inflammation when leukocytes cross the endothelium. Initially described as a receptor for lymphocyte function-associated antigen-1 (LFA1), ICAM2 may play a role in lymphocyte recirculation by blocking LFA-1-dependent cell adhesion. It mediates adhesive interactions important for antigen-specific immune response, NK-cell mediated clearance, lymphocyte recirculation, and other cellular interactions important for immune response and surveillance. ICAM2 has six N-linked glycosylation sites at amino acids (asparagines) 47, 82, 105, 153, 178 and 187. This antibody got 55-80 kDa in western blotting maybe due to glycosylation.

实验方案

Product Specific Protocols
WB protocol for ICAM2 antibody 27322-1-APDownload protocol
FC protocol for ICAM2 antibody 27322-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols