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ICAM3/CD50 Polyclonal antibody

ICAM3/CD50 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17699-1-AP

Print datasheet

Synonyms

CDW50, ICAM3, ICAM-3, ICAM R, ICAM 3

验证数据展示

  • WB analysis of Jurkat using 17699-1-AP

    WB analysis of Jurkat using 17699-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 17699-1-AP (ICAM3/CD50 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 17699-1-AP

    IHC staining of human colon using 17699-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3/CD50 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon using 17699-1-AP

    IHC staining of human colon using 17699-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17699-1-AP (ICAM3 antibody) at dilution of 1:100 (under 40x lens).

  • IP experiment of Jurkat using 17699-1-AP

    IP experiment of Jurkat using 17699-1-AP

    IP result of anti-ICAM3/CD50 (IP:17699-1-AP, 4ug; Detection:17699-1-AP 1:500) with Jurkat cells lysate 4000 ug.

经过测试的应用

Positive WB detected inJurkat cells, Ramos cells, U-937 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17699-1-AP targets ICAM3/CD50 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IP, ELISA Application Description
Tested Reactivity human
Immunogen ICAM3/CD50 fusion protein Ag11955 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name intercellular adhesion molecule 3
Synonyms CDW50, ICAM3, ICAM-3, ICAM R, ICAM 3
Calculated Molecular Weight 547 aa, 60 kDa
Observed Molecular Weight 110-160 kDa, 60 kDa
GenBank Accession NumberBC058903
Gene Symbol ICAM3/CD50
Gene ID (NCBI) 3385
RRIDAB_2248763
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32942
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ICAM3/CD50 antibody 17699-1-APDownload protocol
IHC protocol for ICAM3/CD50 antibody 17699-1-APDownload protocol
IP protocol for ICAM3/CD50 antibody 17699-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols