• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

ICAM3/CD50 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 78.1-5000 pg/mL

Cat No : MP01223-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01223-3, Human ICAM3/CD50 Recombinant Matched Antibody Pair - PBS only. 84335-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02342. 84335-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

    Sandwich ELISA standard curve of MP01223-3

    Sandwich ELISA standard curve of MP01223-3, Human ICAM3/CD50 Recombinant Matched Antibody Pair - PBS only. 84335-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02342. 84335-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

  • Serum of twelve individual healthy human donors was measured. The humam ICAM3/CD50 concentration of detected samples was determined to be 100.57 ng/mL with a range of 80.46 - 116.80 ng/mL.

    Sample test of MP01223-3

    Serum of twelve individual healthy human donors was measured. The humam ICAM3/CD50 concentration of detected samples was determined to be 100.57 ng/mL with a range of 80.46 - 116.80 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

产品信息

MP01223-3 targets ICAM3/CD50 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 78.1-5000 pg/mL (Sandwich ELISA);

Capture antibody: ICAM3 Recombinant antibody, PBS Only (Capture) 84335-4-PBS (241418G8). 100 μg. Concentration 1 mgl/ml.

Detection antibody: ICAM3 Recombinant antibody, PBS Only (Detector) 84335-2-PBS (241417G2). 100 μg. Concentration 1 mgl/ml.

Alternative ICAM3/CD50 matched antibody pairs: MP01223-1, MP01223-2

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84335-4-PBS
Tested Reactivity human
Immunogen Recombinant Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intercellular adhesion molecule 3
Synonyms CDW50, ICAM-3, ICAM R, ICAM 3, CD50
GenBank accession numberBC058903
Gene symbol ICAM3/CD50
UniprotP32942
Gene ID (NCBI) 3385
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
Catalog Number 84335-2-PBS
Tested Reactivity human
Immunogen Recombinant Protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name intercellular adhesion molecule 3
Synonyms CDW50, ICAM-3, ICAM R, ICAM 3, CD50
GenBank accession numberBC058903
Gene symbol ICAM3/CD50
UniprotP32942
Gene ID (NCBI) 3385
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C