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ICAM4 Monoclonal antibody, PBS Only

ICAM4 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3A9B9

Cat no : 67014-1-PBS

Print datasheet

Synonyms

CD242, ICAM 4, ICAM4, ICAM-4, LW, LW blood group protein

验证数据展示

  • WB analysis using 67014-1-Ig (same clone as 67014-1-PBS)

    WB analysis using 67014-1-Ig (same clone as 67014-1-PBS)

    Different amount of human blood lysates were subjected to SDS PAGE followed by western blot with 67014-1-Ig (ICAM4 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ICAM4 antibody included
  • IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    IHC staining of human spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 67014-1-Ig (ICAM4 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ICAM4 antibody included
  • IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    IF Staining of mouse spleen using 67014-1-Ig (same clone as 67014-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using ICAM4 antibody (67014-1-Ig, Clone: 3A9B9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67014-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ICAM4 Ready-To-Use IHC Kit. ICAM4 primary antibody included.

产品信息

67014-1-PBS targets ICAM4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity Human, mouse
Immunogen ICAM4 fusion protein Ag27628 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name intercellular adhesion molecule 4 (Landsteiner-Wiener blood group)
Synonyms CD242, ICAM 4, ICAM4, ICAM-4, LW, LW blood group protein
Calculated Molecular Weight 271 aa, 29 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC029364
Gene Symbol ICAM4
Gene ID (NCBI) 3386
RRIDAB_2882330
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14773
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

This gene encodes the Landsteiner-Wiener (LW) blood group antigen(s) that belongs to the immunoglobulin (Ig) superfamily, and that shares similarity with the intercellular adhesion molecule (ICAM) protein family. The protein migrate as a 42 kDa glycoprotein on SDS PAGE. Alternative splicing results in multiple transcript variants encoding distinct isoforms.