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验证数据展示

  • WB analysis of Jurkat using 13338-1-AP

    WB analysis of Jurkat using 13338-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13338-1-AP (ICOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 13338-1-AP

    IHC staining of human tonsillitis using 13338-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13338-1-AP (ICOS antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of human tonsillitis using 13338-1-AP

    IF Staining of human tonsillitis using 13338-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using ICOS antibody (13338-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ICOS/CD278 Polyclonal antibody

ICOS/CD278 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 13338-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF-P, ELISA

别名

ICOS, inducible T cell co stimulator, CVID1, CD278, AILIM

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, HL-60 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13338-1-AP targets ICOS/CD278 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human
免疫原 ICOS/CD278 fusion protein Ag4155 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inducible T-cell co-stimulator
别名 ICOS, inducible T cell co stimulator, CVID1, CD278, AILIM
计算分子量 199 aa, 23 kDa
观测分子量 28 kDa, 45-50 kDa
GenBank蛋白编号BC028210
基因名称 ICOS
Gene ID (NCBI) 29851
RRIDAB_2122590
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6W8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inducible T cell co-stimulator (ICOS) is related to the CD28 superfamily and is highly expressed on activated T cells as well as regulatory T cells and is crucial for the survival and function of T cells, Th2 cell differentiation, and lung inflammatory responses. Binding ICOS to ICOS-ligand (ICOS-L) activates a cascade of intracellular signaling molecules that prevent apoptosis and lead to the production of cytokines such as IL-4 and IL-13. Catalog#13338-1-AP recognizes two bands of 25-30 kDa and 45-50 kDa, and the additional 45-50 kDa band may be due to  surface disulfide-linked homodimeric glycoprotein.

实验方案

Product Specific Protocols
WB protocol for ICOS/CD278 antibody 13338-1-APDownload protocol
IHC protocol for ICOS/CD278 antibody 13338-1-APDownload protocol
IF protocol for ICOS/CD278 antibody 13338-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols