ICOSLG/B7-H2/CD275 Polyclonal antibody
ICOSLG/B7-H2/CD275 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Daudi cells, JAR cells |
Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
IHC | See 2 publications below |
产品信息
14922-1-AP targets ICOSLG/B7-H2/CD275 in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | IHC |
Tested Reactivity | human |
Cited Reactivity | human |
Immunogen | ICOSLG/B7-H2/CD275 fusion protein Ag6392 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | inducible T-cell co-stimulator ligand |
Synonyms | B7H2, ICOSLG, B7 H2, B7RP-1, B7RP1 |
Calculated Molecular Weight | 33 kDa, 34 kDa |
Observed Molecular Weight | 60-70 kDa |
GenBank Accession Number | BC064637 |
Gene Symbol | ICOSLG |
Gene ID (NCBI) | 23308 |
RRID | AB_2122732 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O75144 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ICOSLG/B7-H2/CD275 antibody 14922-1-AP | Download protocol |
IHC protocol for ICOSLG/B7-H2/CD275 antibody 14922-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cancers (Basel) Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models. | ||
Cell Mol Gastroenterol Hepatol Sex Differences in Genomic Features of Hepatitis B-Associated Hepatocellular Carcinoma With Distinct Antitumor Immunity |