验证数据展示
经过测试的应用
| Positive WB detected in | A2780 cells, SKOV-3 cells |
| Positive IP detected in | SKOV-3 cells |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 7 publications below |
产品信息
10403-1-AP targets ICT1 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | ICT1 fusion protein Ag0647 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | immature colon carcinoma transcript 1 |
| 别名 | MRPL58, Large ribosomal subunit protein mL62, EC:3.1.1.29, DS-1, DS1 |
| 计算分子量 | 24 kDa |
| 观测分子量 | 21-24 kDa |
| GenBank蛋白编号 | BC015335 |
| 基因名称 | ICT1 |
| Gene ID (NCBI) | 3396 |
| RRID | AB_2122853 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q14197 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The adult colon epithelium contains 3 differentiated cell types that arise from a multipotent stem cell. Deviation from the normal maturation pathway by neoplastic transformation is thought to initiate in stem cells or their early descendants. One potential marker is immature colon carcinoma transcript 1 (ICT1, synonym: DS-1) whose mRNA and protein were more highly expressed in undifferentiated than differentiated cells. This antibody is a rabbit polyclonal antibody raised against a full-length human ICT1 protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ICT1 antibody 10403-1-AP | Download protocol |
| IF protocol for ICT1 antibody 10403-1-AP | Download protocol |
| IP protocol for ICT1 antibody 10403-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep The Human Mitochondrial DEAD-Box Protein DDX28 Resides in RNA Granules and Functions in Mitoribosome Assembly. | ||
PLoS Genet RCC1L (WBSCR16) isoforms coordinate mitochondrial ribosome assembly through their interaction with GTPases. | ||
Mitochondrion Interactions between peptidyl tRNA hydrolase homologs and the ribosomal release factor Mrf1 in S. pombe mitochondria. | ||
Biochem J The human RNA-binding protein RBFA promotes the maturation of the mitochondrial ribosome. |
