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ID1 Monoclonal antibody, PBS Only

ID1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1H3G11

Cat no : 67827-1-PBS

Print datasheet

Synonyms

bHLHb24, ID, ID1, Inhibitor of DNA binding 1

验证数据展示

  • WB analysis using 67827-1-Ig (same clone as 67827-1-PBS)

    WB analysis using 67827-1-Ig (same clone as 67827-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67827-1-Ig (ID1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67827-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67827-1-Ig (same clone as 67827-1-PBS)

    IHC staining of human lung cancer using 67827-1-Ig (same clone as 67827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67827-1-Ig (ID1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67827-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ID1 antibody included
Planning an IHC experiment? We recommend our IHCeasy ID1 Ready-To-Use IHC Kit. ID1 primary antibody included.

产品信息

67827-1-PBS targets ID1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen ID1 fusion protein Ag13359 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
Synonyms bHLHb24, ID, ID1, Inhibitor of DNA binding 1
Calculated Molecular Weight 16 kDa
Observed Molecular Weight18 kDa
GenBank Accession NumberBC000613
Gene Symbol ID1
Gene ID (NCBI) 3397
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP41134
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 is about 18 kDa.