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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 67827-1-Ig (same clone as 67827-1-PBS)

    WB analysis using 67827-1-Ig (same clone as 67827-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67827-1-Ig (ID1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67827-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 67827-1-Ig (same clone as 67827-1-PBS)

    IHC staining of human lung cancer using 67827-1-Ig (same clone as 67827-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67827-1-Ig (ID1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67827-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ID1 antibody included

ID1 Monoclonal antibody, PBS Only

ID1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 67827-1-PBS

产品说明书

CloneNo. 1H3G11

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, rat

应用

WB, IHC, ELISA

别名

bHLHb24, ID, ID1, Inhibitor of DNA binding 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ID1 Ready-To-Use IHC Kit. ID1 primary antibody included.

产品信息

67827-1-PBS targets ID1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 ID1 fusion protein Ag13359 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 inhibitor of DNA binding 1, dominant negative helix-loop-helix protein
别名 bHLHb24, ID, ID1, Inhibitor of DNA binding 1
计算分子量 16 kDa
观测分子量18 kDa
GenBank蛋白编号BC000613
基因名称 ID1
Gene ID (NCBI) 3397
RRIDAB_2918589
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP41134
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ID (inhibitor of DNA binding) proteins contain a helix-loop-helix (HLH) motif and regulate tissue-specific transcription within several cell lineages. They do not bind DNA directly, but inhibit lineage commitment by binding basic helix-loop-helix (bHLH) transcription factors through their HLH motif. ID1 proteins lack a basic DNA-binding domain but are able to form heterodimers with other HLH proteins, thereby inhibiting DNA binding. ID proteins contribute to cell growth, senescence, differentiation, and angiogenesis. Inhibitor of differentiation or DNA binding-1 (ID1) interacts with the transactivation domain of p65 (p65TAD) both in vitro and in vivo. The molecular weight of ID1 is about 18 kDa.