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IDH1 Polyclonal antibody, PBS Only

IDH1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 23309-1-PBS

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Synonyms

验证数据展示

  • WB analysis using 23309-1-AP (same clone as 23309-1-PBS)

    WB analysis using 23309-1-AP (same clone as 23309-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23309-1-AP (IDH1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23309-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 23309-1-AP (same clone as 23309-1-PBS)

    IHC staining of mouse kidney using 23309-1-AP (same clone as 23309-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 23309-1-AP (IDH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23309-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH1 antibody included
Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.

产品信息

23309-1-PBS targets IDH1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IDH1 fusion protein Ag19749 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Synonyms
Calculated Molecular Weight 414 aa, 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC012846
Gene Symbol IDH1
Gene ID (NCBI) 3417
RRIDAB_11232028
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75874
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. Its subunit structure is homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ). This antibody is specific to IDH1.