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IDH1 Monoclonal antibody, PBS Only

IDH1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A6A2

Cat no : 66197-1-PBS

Print datasheet

Synonyms

IDH 1, IDH, IDCD, EC:1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase

验证数据展示

  • WB analysis of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    WB analysis of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66197-1-Ig (IDH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66197-1-Ig (same clone as 66197-1-PBS)

    IHC staining of human liver cancer using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66197-1-Ig (IDH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH1 antibody included
  • IHC staining of human liver cancer using 66197-1-Ig (same clone as 66197-1-PBS)

    IHC staining of human liver cancer using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66197-1-Ig (IDH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH1 antibody included
  • IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66197-1-Ig (IDH1 antibody) at dilution of 1:400 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    IF Staining of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde ) fixed HepG2 cells using 66197-1-Ig(IDH1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    FC experiment of HepG2 using 66197-1-Ig (same clone as 66197-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug IDH1 Monoclonal antibody (66197-1-Ig, Clone:2A6A2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66197-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy IDH1 Ready-To-Use IHC Kit. IDH1 primary antibody included.

产品信息

66197-1-PBS targets IDH1 in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen IDH1 fusion protein Ag19293 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Synonyms IDH 1, IDH, IDCD, EC:1.1.1.42, Cytosolic NADP-isocitrate dehydrogenase
Calculated Molecular Weight 414 aa, 47 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC012846
Gene Symbol IDH1
Gene ID (NCBI) 3417
RRIDAB_2881590
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75874
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IDH1, also named as PICD and IDP, belongs to the isocitrate and isopropylmalate dehydrogenases family. It is a common feature of a major subset of primary human brain cancers. It can form a homodimer(PMID:15173171).IDH1 mutation is always heterozygotic and IDH1 functions as a dimer, theoretically there will be 25% each wild type and mutant homo-dimers and 50% hetero-dimers present in the tumor cells(PMID:21079649 ).