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验证数据展示

  • WB analysis using 23254-1-AP (same clone as 23254-1-PBS)

    WB analysis using 23254-1-AP (same clone as 23254-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23254-1-AP (IDH2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 23254-1-AP (same clone as 23254-1-PBS)

    IHC staining of human heart using 23254-1-AP (same clone as 23254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23254-1-AP (IDH2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH2 antibody included
  • IHC staining of human heart using 23254-1-AP (same clone as 23254-1-PBS)

    IHC staining of human heart using 23254-1-AP (same clone as 23254-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart slide using 23254-1-AP (IDH2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDH2 antibody included
  • IF Staining of HepG2 using 23254-1-AP (same clone as 23254-1-PBS)

    IF Staining of HepG2 using 23254-1-AP (same clone as 23254-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23254-1-AP (IDH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23254-1-PBS in a different storage buffer formulation.

IDH2 Polyclonal antibody, PBS Only

IDH2 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 23254-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IDH2 Ready-To-Use IHC Kit. IDH2 primary antibody included.

产品信息

23254-1-PBS targets IDH2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IDH2 fusion protein Ag19748 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 isocitrate dehydrogenase 2 (NADP+), mitochondrial
别名
计算分子量 452 aa, 51 kDa
观测分子量 41-47 kDa
GenBank蛋白编号BC009244
基因名称 IDH2
Gene ID (NCBI) 3418
RRIDAB_2879241
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48735
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IDH2, also named as IDP and ICD-M, belongs to the isocitrate and isopropylmalate dehydrogenases family. It plays a role in intermediary metabolism and energy production. IDH2 is a mitochondrial NADP-dependent isocitrate dehydrogenase that catalyzes oxidative decarboxylation of isocitrate to alpha-ketoglutarate, producing NADPH. It may tightly associate or interact with the pyruvate dehydrogenase complex. IDH1 and IDH2 mutations could also contribute to tumorigenesis and cancer progression through increased mutagenesis. IDH2 has 2 isoforms with the MW of 51 kDa and 45 kDa, and the mature form is about 47 kDa and 41 kDa with the N-terminal transit peptide cleaved. This antibody is specific to IDH2.