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IDH3G Polyclonal antibody

IDH3G Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25848-1-AP

Print datasheet

Synonyms

NAD(+)-specific ICDH subunit gamma, Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, H IDHG

验证数据展示

  • WB analysis of mouse colon using 25848-1-AP

    WB analysis of mouse colon using 25848-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 25848-1-AP (IDH3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 25848-1-AP

    IHC staining of human skin cancer using 25848-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin cancer using 25848-1-AP

    IHC staining of human skin cancer using 25848-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 25848-1-AP (IDH3G Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human colon using 25848-1-AP

    IHC staining of human colon using 25848-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25848-1-AP (IDH3G antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse colon tissue, A375 cells, A431 cells
Positive IHC detected inhuman colon tissue, human skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

25848-1-AP targets IDH3G in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen IDH3G fusion protein Ag23113 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name isocitrate dehydrogenase 3 (NAD+) gamma
Synonyms NAD(+)-specific ICDH subunit gamma, Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial, H IDHG
Calculated Molecular Weight 43 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC000933
Gene Symbol IDH3G
Gene ID (NCBI) 3421
RRIDAB_2880267
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51553
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IDH3G antibody 25848-1-APDownload protocol
IHC protocol for IDH3G antibody 25848-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Oncogene

Hypoxia-reprogrammed tricarboxylic acid cycle promotes the growth of human breast tumorigenic cells.

Authors - Ke Tang
View Article