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IDO1 Monoclonal antibody, PBS Only

IDO1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G2G11

Cat no : 66528-1-PBS

Print datasheet

Synonyms

INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52

验证数据展示

  • WB analysis using 66528-1-Ig (same clone as 66528-1-PBS)

    WB analysis using 66528-1-Ig (same clone as 66528-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66528-1-Ig (same clone as 66528-1-PBS)

    IHC staining of human tonsillitis using 66528-1-Ig (same clone as 66528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66528-1-Ig (IDO1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDO1 antibody included
  • IF Staining of SKOV-3 using 66528-1-Ig (same clone as 66528-1-PBS)

    IF Staining of SKOV-3 using 66528-1-Ig (same clone as 66528-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using IDO1 antibody (66528-1-Ig, Clone: 3G2G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy IDO1 Ready-To-Use IHC Kit. IDO1 primary antibody included.

产品信息

66528-1-PBS targets IDO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IDO1 fusion protein Ag3953 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name indoleamine 2,3-dioxygenase 1
Synonyms INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52
Calculated Molecular Weight 403 aa, 45 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC027882
Gene Symbol IDO1
Gene ID (NCBI) 3620
RRIDAB_2881891
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14902
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).