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验证数据展示

  • WB analysis using 66528-1-Ig (same clone as 66528-1-PBS)

    WB analysis using 66528-1-Ig (same clone as 66528-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66528-1-Ig (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66528-1-Ig (same clone as 66528-1-PBS)

    IHC staining of human tonsillitis using 66528-1-Ig (same clone as 66528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66528-1-Ig (IDO1 antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with IDO1 antibody included
  • IF Staining of SKOV-3 using 66528-1-Ig (same clone as 66528-1-PBS)

    IF Staining of SKOV-3 using 66528-1-Ig (same clone as 66528-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using IDO1 antibody (66528-1-Ig, Clone: 3G2G11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66528-1-PBS in a different storage buffer formulation.

IDO1 Monoclonal antibody, PBS Only

IDO1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66528-1-PBS

产品说明书

CloneNo. 3G2G11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy IDO1 Ready-To-Use IHC Kit. IDO1 primary antibody included.

产品信息

66528-1-PBS targets IDO1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 IDO1 fusion protein Ag3953 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 indoleamine 2,3-dioxygenase 1
别名 INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52
计算分子量 403 aa, 45 kDa
观测分子量 42 kDa
GenBank蛋白编号BC027882
基因名称 IDO1
Gene ID (NCBI) 3620
RRIDAB_2881891
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP14902
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).