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IDO1 Recombinant antibody

IDO1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241110F2

Cat no : 84063-3-RR

Print datasheet

Synonyms

INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52

验证数据展示

  • WB analysis using 84063-3-RR

    WB analysis using 84063-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84063-3-RR (IDO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 84063-3-RR

    IHC staining of mouse brain using 84063-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84063-3-RR (IDO1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IDO1 antibody included
  • Affinity and Kinetic Characterization of 84063-3-RR

    Affinity and Kinetic Characterization of 84063-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84063-3-RR against Human IDO1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inrat lung tissue, human plancta tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IDO1 Ready-To-Use IHC Kit. IDO1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84063-3-RR targets IDO1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen IDO1 fusion protein Ag3953 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name indoleamine 2,3-dioxygenase 1
Synonyms INDO, IDO-1, IDO 1, IDO, EC:1.13.11.52
Calculated Molecular Weight 403 aa, 45 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberBC027882
Gene Symbol IDO1
Gene ID (NCBI) 3620
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP14902
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IDO1 is the target for therapy in a range of clinical settings, including cancer, chronic infections, autoimmune and allergic syndromes, and transplantation. Elevated IDO1 expression is a hallmark of major viral infections including HIV, HBV, HCV or influenza and also of major bacteria infections, such as Tb, CAP, listeriosis and sepsis. Pathogens are able to highjack the immunosuppressive effects of IDO1 and make use of them to facilitate their own life cycle. MW of IDO1 is 40-42kd (PMID: 14502282; 17055065).

实验方案

Product Specific Protocols
WB protocol for IDO1 antibody 84063-3-RRDownload protocol
IHC protocol for IDO1 antibody 84063-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols