IDS Recombinant antibody
IDS Uni-rAbTM Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
242054A7
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, HepG2 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84623-6-RR targets IDS in WB, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, ELISA Application Description |
| Tested Reactivity | human |
| Immunogen | IDS fusion protein Ag31132 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | iduronate 2-sulfatase |
| Synonyms | Iduronate 2-sulfatase 42 kDa chain, Iduronate 2-sulfatase 14 kDa chain, Iduronate 2-sulfatase, EC:3.1.6.13, Alpha-L-iduronate sulfate sulfatase |
| Calculated Molecular Weight | 312aa,35 kDa; 550aa,62 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC006170 |
| Gene Symbol | IDS |
| Gene ID (NCBI) | 3423 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P22304 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Iduronate 2-sulfatase (IDS) is required for the lysosomal degradation of heparan sulfate and dermatan sulfate. Mutations causing IDS deficiency in humans result in the lysosomal storage of these glycosaminoglycans and Hunter syndrome, an X chromosome-linked disease. IDS is synthesized as two precursor forms of 76 and 90 kDa that are converted, through a 62 kDa intermediate, to mature polypeptides due to an internal proteolytic cleavage (PMID: 10838181).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IDS antibody 84623-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |