IDUA Polyclonal antibody
IDUA Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55158-1-AP targets IDUA in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IP, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | iduronidase, alpha-L- |
| Synonyms | Alpha L iduronidase, IDA, IDUA, iduronidase, alpha L, MPS1 |
| Calculated Molecular Weight | 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | NM_000203 |
| Gene Symbol | IDUA |
| Gene ID (NCBI) | 3425 |
| RRID | AB_11182946 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35475 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IDUA belongs to the glycosyl hydrolase 39 family. It hydrolysis of unsulfated alpha-L-iduronosidic linkages in dermatan sulfate. Defects in IDUA are the cause of mucopolysaccharidosis type 1H (MPS1H)( also known as Hurler syndrome), MPS1H/S(also known as Hurler-Scheie syndrome) and MPS1S(also known as Scheie syndrome), which are rare lysosomal storage diseases. This antibody is specific to IDUA.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for IDUA antibody 55158-1-AP | Download protocol |
| IHC protocol for IDUA antibody 55158-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Ther Methods Clin Dev Liver-directed gene therapy corrects neurologic disease in a murine model of mucopolysaccharidosis type I-Hurler. | ||
Mol Cell Biochem Why SNP rs3755955 is associated with human bone mineral density? A molecular and cellular study in bone cells. | ||
Front Immunol Leveraging diverse cell-death related signature predicts the prognosis and immunotherapy response in renal clear cell carcinoma |
