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IER3IP1 Polyclonal antibody

IER3IP1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29402-1-AP

Print datasheet

Synonyms

HSPC039, IER3IP1, PRO2309

验证数据展示

  • WB analysis using 29402-1-AP

    WB analysis using 29402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29402-1-AP (IER3IP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 29402-1-AP

    IHC staining of human kidney using 29402-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29402-1-AP (IER3IP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U2OS using 29402-1-AP

    IF Staining of U2OS using 29402-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using IER3IP1 antibody (29402-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29402-1-AP targets IER3IP1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen IER3IP1 fusion protein Ag28027 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name immediate early response 3 interacting protein 1
Synonyms HSPC039, IER3IP1, PRO2309
Calculated Molecular Weight 82 aa, 9 kDa
Observed Molecular Weight9 kDa
GenBank Accession NumberBC010888
Gene Symbol IER3IP1
Gene ID (NCBI) 51124
RRIDAB_2918295
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y5U9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IER3IP1 antibody 29402-1-APDownload protocol
IHC protocol for IER3IP1 antibody 29402-1-APDownload protocol
IF protocol for IER3IP1 antibody 29402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols