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验证数据展示

  • WB analysis using 29280-1-AP

    WB analysis using 29280-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29280-1-AP (IFI16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 29280-1-AP

    IHC staining of human renal cell carcinoma using 29280-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29280-1-AP (IFI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFI16 antibody included

IFI16 Polyclonal antibody

IFI16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 29280-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, IF/ICC, ELISA

别名

Ifi 16, IFI16, IFNGIP1, PYHIN2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, Jurkat cells, MOLT-4 cells, Ramos cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IFI16 Ready-To-Use IHC Kit. IFI16 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29280-1-AP targets IFI16 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 Human
免疫原 IFI16 fusion protein Ag30297 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon, gamma-inducible protein 16
别名 Ifi 16, IFI16, IFNGIP1, PYHIN2
计算分子量 729 aa, 82 kDa
观测分子量85-95 kDa
GenBank蛋白编号BC017059
基因名称 IFI16
Gene ID (NCBI) 3428
RRIDAB_3086109
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ16666
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)

实验方案

Product Specific Protocols
WB protocol for IFI16 antibody 29280-1-APDownload protocol
IHC protocol for IFI16 antibody 29280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols