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IFI16 Polyclonal antibody

IFI16 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 29280-1-AP

Print datasheet

Synonyms

Ifi 16, IFI16, IFNGIP1, PYHIN2

验证数据展示

  • WB analysis using 29280-1-AP

    WB analysis using 29280-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29280-1-AP (IFI16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human renal cell carcinoma using 29280-1-AP

    IHC staining of human renal cell carcinoma using 29280-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29280-1-AP (IFI16 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFI16 antibody included

经过测试的应用

Positive WB detected inDaudi cells, Jurkat cells, MOLT-4 cells, Ramos cells
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IFI16 Ready-To-Use IHC Kit. IFI16 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29280-1-AP targets IFI16 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IHC, WB, ELISA Application Description
Tested Reactivity Human
Immunogen IFI16 fusion protein Ag30297 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon, gamma-inducible protein 16
Synonyms Ifi 16, IFI16, IFNGIP1, PYHIN2
Calculated Molecular Weight 729 aa, 82 kDa
Observed Molecular Weight85-95 kDa
GenBank Accession NumberBC017059
Gene Symbol IFI16
Gene ID (NCBI) 3428
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16666
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFI16 is a member of a family of interferon-inducible nuclear proteins involved in transcriptional regulation functions as a transcriptional repressor. IFI16 may be involved in TP53-mediated transcriptional activation by enhancing TP53 sequence-specific DNA binding and modulating TP53 phosphorylation status. It has been reported that IFI16 participates in the regulation of autophagy, TP53-mediated cell death and innate immune response. IFI16 has different isoforms and 67790-1-Ig antibody detects proteins around 85-95 kDa in SDS-PAGE similar to papers. (PMID: 9718316, 14990579, 11146555, 22046441)

实验方案

Product Specific Protocols
WB protocol for IFI16 antibody 29280-1-APDownload protocol
IHC protocol for IFI16 antibody 29280-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols