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  • Featured Product
  • KD/KO Validated

IFI30 Polyclonal antibody

IFI30 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11597-1-AP

Print datasheet

Synonyms

IP30, IFI 30, GILT, Gamma-interferon-inducible protein IP-30, Gamma-interferon-inducible lysosomal thiol reductase

验证数据展示

  • IP experiment of COLO 320 using 11597-1-AP

    IP experiment of COLO 320 using 11597-1-AP

    IP result of anti-IFI30 (IP:11597-1-AP, 4ug; Detection:11597-1-AP 1:3000) with COLO 320 cells lysate 2400 ug.

  • IF Staining of HeLa using 11597-1-AP

    IF Staining of HeLa using 11597-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 11597-1-AP (IFI30 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis of COLO 320 using 11597-1-AP

    WB analysis of COLO 320 using 11597-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 11597-1-AP (IFI30 Antibody) at dilution of 1:6000 incubated at 4 degree celsius over night.

  • IHC staining of human liver cancer using 11597-1-AP

    IHC staining of human liver cancer using 11597-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11597-1-AP (IFI30 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFI30 antibody included
  • IHC staining of human liver cancer using 11597-1-AP

    IHC staining of human liver cancer using 11597-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11597-1-AP (IFI30 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFI30 antibody included

经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy IFI30 Ready-To-Use IHC Kit. IFI30 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11597-1-AP targets IFI30 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human
Cited Reactivityhuman
Immunogen IFI30 fusion protein Ag2178 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon, gamma-inducible protein 30
Synonyms IP30, IFI 30, GILT, Gamma-interferon-inducible protein IP-30, Gamma-interferon-inducible lysosomal thiol reductase
Calculated Molecular Weight 261 aa, 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC021136
Gene Symbol IFI30
Gene ID (NCBI) 10437
RRIDAB_2121789
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP13284
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFI30(interferon, gamma-inducible protein 30) is also named as IP30, GILT, IFI-30, MGC32056, IFI30 and belongs to the GILT family. It can facilitate the processing and presentation to antigen-specific T cells of protein antigens containing disulfide bonds. IFI30 is inducibly up-regulated by IFN-g, as are MHC class II molecules, the associated invariant chain, and HLA-DM, which catalyses peptide loading in MIICs and the 35 kDa species is the IFI30 proform, and the 30-kDa species is the mature form(PMID:10639150). It can exsit as a dimer(PMID:17142755).

实验方案

Product Specific Protocols
WB protocol for IFI30 antibody 11597-1-APDownload protocol
IHC protocol for IFI30 antibody 11597-1-APDownload protocol
IF protocol for IFI30 antibody 11597-1-APDownload protocol
IP protocol for IFI30 antibody 11597-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Int J Med Sci

IFI30 expression predicts patient prognosis in breast cancer and dictates breast cancer cells proliferation via regulating autophagy.

Authors - Yan Fan
  • KD Validated
View Article
humanWB

Phytomedicine

Nobiletin inhibits de novo FA synthesis to alleviate gastric cancer progression by regulating endoplasmic reticulum stress

Authors - Menglin Chen
View Article
humanWB

Aging (Albany NY)

Identification and validation of a novel signature based on macrophage marker genes for predicting prognosis and drug response in kidney renal clear cell carcinoma by integrated analysis of single cell and bulk RNA sequencing

Authors - Xiaoxu Chen
View Article