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验证数据展示

  • IF Staining of HeLa using 83160-2-RR (same clone as 83160-2-PBS)

    IF Staining of HeLa using 83160-2-RR (same clone as 83160-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IFI35 antibody (83160-2-RR, Clone: 240063C6 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83160-2-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83160-2-RR (same clone as 83160-2-PBS)

    FC experiment of A549 using 83160-2-RR (same clone as 83160-2-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug Ifi35 Recombinant Antibody (83160-2-RR, Clone:240063C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83160-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83160-2-RR (same clone as 83160-2-PBS)

    FC experiment of HeLa using 83160-2-RR (same clone as 83160-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ifi35 Recombinant Antibody (83160-2-RR, Clone:240063C6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83160-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00191-2

    Cytometric bead array standard curve of MP00191-2

    Cytometric bead array standard curve of MP00191-2, IFI35 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83160-2-PBS. Detection antibody: 83160-1-PBS. Standard: Ag34653. Range: 0.625-20 ng/mL.

  • Affinity and Kinetic Characterization of 83160-2-PBS

    Affinity and Kinetic Characterization of 83160-2-PBS

    Biolayer interferometry (BLl) kinetic assay of 83160-2-PBS against Human IFI35 was performed. The affinity constant is 1.93 nM.

  • Affinity and Kinetic Characterization of 83160-2-RR

    Affinity and Kinetic Characterization of 83160-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83160-2-RR against Human IFI35 were performed. The affinity constant is below 1 pM.

IFI35 Recombinant antibody, PBS Only (Capture)

IFI35 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83160-2-PBS

产品说明书

CloneNo. 240063C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

别名

interferon induced protein 35, IFP35, IFP 35, Ifi-35, Ifi 35

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

83160-2-PBS targets IFI35 as part of a matched antibody pair:

MP00191-2: 83160-2-PBS capture and 83160-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 IFI35 fusion protein Ag34653 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interferon-induced protein 35
别名 interferon induced protein 35, IFP35, IFP 35, Ifi-35, Ifi 35
计算分子量 31 kDa
GenBank蛋白编号BC001356
基因名称 IFI35
Gene ID (NCBI) 3430
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP80217
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

IFI35 (interferon induced protein 35), also known as IFP35. It is expected to be located in cytoplasm, nucleus and extracellular space. The protein is expressed in a wide range of cell types, including fibroblasts, macrophages, and epithelial cells. Involved in several processes, including macrophage activation involved in immune response; positive regulation of defense response; and regulation of signal transduction. And the calculated molecular weight of IFI35 is 31 kDa.