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验证数据展示

  • WB analysis using 83424-1-RR

    WB analysis using 83424-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83424-1-RR (IFI44 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 83424-1-RR

    FC experiment of A549 using 83424-1-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug IFI44 Recombinant antibody (83424-1-RR, Clone:240022F12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83424-1-RR

    Affinity and Kinetic Characterization of 83424-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83424-1-RR against Human IFI44 were performed. The affinity constant is 5.57 nM.

IFI44 Recombinant antibody

IFI44 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83424-1-RR

产品说明书

CloneNo. 240022F12

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

p44, MTAP44, Microtubule-associated protein 44, Interferon-induced protein 44, interferon induced protein 44

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, MCF-7 cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83424-1-RR targets IFI44 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 IFI44 fusion protein Ag26121 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 interferon-induced protein 44
别名 p44, MTAP44, Microtubule-associated protein 44, Interferon-induced protein 44, interferon induced protein 44
计算分子量 444 aa, 50 kDa
观测分子量 50 kDa
GenBank蛋白编号BC022870
基因名称 IFI44
Gene ID (NCBI) 10561
RRIDAB_3671073
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ8TCB0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for IFI44 antibody 83424-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols