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验证数据展示

  • WB analysis using 16879-1-AP

    WB analysis using 16879-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16879-1-AP (IFIH1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 16879-1-AP

    IHC staining of human ovary cancer using 16879-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16879-1-AP (IFIH1-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFIH1 antibody included

IFIH1-Specific Polyclonal antibody

IFIH1-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16879-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

IFIH1, MDA5, IFIH 1, Helicard, EC:3.6.4.13

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated

规格: 
50 μL
100 μL
150 μL

¥2200/ 100 μL

浓度: 260 ug/ml

库存：现货

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IFIH1 Ready-To-Use IHC Kit. IFIH1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16879-1-AP targets IFIH1-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interferon induced with helicase C domain 1
别名 IFIH1, MDA5, IFIH 1, Helicard, EC:3.6.4.13
计算分子量 117 kDa
观测分子量70 kDa, 90 kDa
GenBank蛋白编号NM_022168
基因名称 IFIH1
Gene ID (NCBI) 64135
RRIDAB_3085510
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BYX4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501). This antibody is specific to IFIH1.

实验方案

Product Specific Protocols
WB protocol for IFIH1-Specific antibody 16879-1-APDownload protocol
IHC protocol for IFIH1-Specific antibody 16879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols