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验证数据展示

  • WB analysis using 16879-1-AP

    WB analysis using 16879-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16879-1-AP (IFIH1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 16879-1-AP

    IHC staining of human ovary cancer using 16879-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 16879-1-AP (IFIH1-Specific antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with IFIH1 antibody included

IFIH1-Specific Polyclonal antibody

IFIH1-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16879-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

IFIH1, MDA5, IFIH 1, Helicard, EC:3.6.4.13

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy IFIH1 Ready-To-Use IHC Kit. IFIH1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16879-1-AP targets IFIH1-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon induced with helicase C domain 1
Synonyms IFIH1, MDA5, IFIH 1, Helicard, EC:3.6.4.13
Calculated Molecular Weight 117 kDa
Observed Molecular Weight70 kDa, 90 kDa
GenBank Accession NumberNM_022168
Gene Symbol IFIH1
Gene ID (NCBI) 64135
RRIDAB_3085510
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BYX4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIH1 (Interferon-induced helicase C domain-containing protein 1) is a putative RNA helicase that is upregulated in response to treatment with IFNB or IFNB and MEZ. It is also named as MDA5 and RH116. Ectopic expression of MDA5 in melanoma cells resulted in reduced colony formation, suggesting an interaction of the CARD and apoptotic signal molecules. Functional analysis indicated that MDA5 is an RNA-dependent ATPase. IFIH1 has an apparent molecular mass of 117-130 kDa, and always other bands (70 kDa and 90 kDa) can be detected as cleaved products (PMID: 17267501). This antibody is specific to IFIH1.

实验方案

Product Specific Protocols
WB protocol for IFIH1-Specific antibody 16879-1-APDownload protocol
IHC protocol for IFIH1-Specific antibody 16879-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols