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IFIT1 Polyclonal antibody

IFIT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29897-1-AP

Print datasheet

Synonyms

IFIT-1, IFIT 1, IFI-56K, IFI56, IFI 56K

验证数据展示

  • WB analysis using 29897-1-AP

    WB analysis using 29897-1-AP

    untreated and IFN alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 29897-1-AP (IFIT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 29897-1-AP

    IHC staining of human ovary cancer using 29897-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 29897-1-AP (IFIT1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inIFN alpha treated HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29897-1-AP targets IFIT1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen IFIT1 fusion protein Ag31980 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name interferon-induced protein with tetratricopeptide repeats 1
Synonyms IFIT-1, IFIT 1, IFI-56K, IFI56, IFI 56K
Calculated Molecular Weight 478 aa, 55 kDa
Observed Molecular Weight 55 KDa
GenBank Accession NumberBC007091
Gene Symbol IFIT1
Gene ID (NCBI) 3434
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP09914
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.

实验方案

Product Specific Protocols
WB protocol for IFIT1 antibody 29897-1-APDownload protocol
IHC protocol for IFIT1 antibody 29897-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols