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IFIT1 Recombinant antibody

IFIT1 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240132G11

Cat no : 83423-1-RR

Print datasheet

Synonyms

IFIT 1, IFI-56K, IFI56, IFI 56K, G10P1

验证数据展示

  • WB analysis of HeLa using 83423-1-RR

    WB analysis of HeLa using 83423-1-RR

    IFN alpha treated HeLa cells were subjected to SDS PAGE followed by western blot with 83423-1-RR (IFIT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 83423-1-RR

    IHC staining of human intrahepatic cholangiocarcinoma using 83423-1-RR

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 83423-1-RR (IFIT1 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 83423-1-RR

    FC experiment of HeLa using 83423-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Ifit1 Recombinant Antibody (83423-1-RR, Clone:240132G11) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83423-1-RR

    Affinity and Kinetic Characterization of 83423-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83423-1-RR against Human IFIT1 were performed. The affinity constant is 0.397 nM.

经过测试的应用

Positive WB detected inIFN alpha treated HeLa cells, SGC-7901 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83423-1-RR targets IFIT1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen IFIT1 fusion protein Ag19691 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interferon-induced protein with tetratricopeptide repeats 1
Synonyms IFIT 1, IFI-56K, IFI56, IFI 56K, G10P1
Calculated Molecular Weight 478 aa, 55 kDa
Observed Molecular Weight 55-65 kDa
GenBank Accession NumberBC007091
Gene Symbol IFIT1
Gene ID (NCBI) 3434
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP09914
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFIT1, also known as glucocorticoid-attenuated response gene 16 protein (GARG-16), is a 463 amino acid protein belonging to the IFIT family. IFIT genes comprise a large family with three (IFIT1, IFIT2, and IFIT3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT1 specifically bound virus PPP-RNA forms a complex with IFIT2 and IFIT3 to sequester the viral PPP-RNA and prevent virus replication.

实验方案

Product Specific Protocols
WB protocol for IFIT1 antibody 83423-1-RRDownload protocol
IHC protocol for IFIT1 antibody 83423-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols