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验证数据展示

  • WB analysis using 83455-5-RR

    WB analysis using 83455-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83455-5-RR (IFITM2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • FC experiment of MCF-7 using 83455-5-RR

    FC experiment of MCF-7 using 83455-5-RR

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug IFITM2 Recombinant antibody (83455-5-RR, Clone:240318B6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 83455-5-RR

    IF Staining of HepG2 using 83455-5-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using IFITM2 antibody (83455-5-RR, Clone: 240318B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of HepG2 using 83455-5-RR

    IF Staining of HepG2 using 83455-5-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IFITM2 antibody (83455-5-RR, Clone: 240318B6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83455-5-RR

    Affinity and Kinetic Characterization of 83455-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83455-5-RR against Human IFITM2 were performed. The affinity constant is below 1 pM.

IFITM2 Recombinant antibody

IFITM2 Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83455-5-RR

Print datasheet

CloneNo. 240318B6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

Interferon-induced transmembrane protein 2, IFITM 2, DSPA2c, 240318B6, 18D

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, SGC-7901 cells, MCF-7 cells, A549 cells
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy IFITM2 Ready-To-Use IHC Kit. IFITM2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83455-5-RR targets IFITM2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen IFITM2 fusion protein Ag3451 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interferon induced transmembrane protein 2 (1-8D)
Synonyms Interferon-induced transmembrane protein 2, IFITM 2, DSPA2c, 240318B6, 18D
Calculated Molecular Weight 132 aa, 15 kDa
Observed Molecular Weight 15 kDa
GenBank Accession NumberBC009696
Gene Symbol IFITM2
Gene ID (NCBI) 10581
RRIDAB_3671091
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ01629
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in a p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also, they serve as important components of the innate immune system to restrict HIV-1 infection.

实验方案

Product Specific Protocols
WB protocol for IFITM2 antibody 83455-5-RRDownload protocol
IF protocol for IFITM2 antibody 83455-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols