验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HepG2 cells, SGC-7901 cells, MCF-7 cells, A549 cells |
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | MCF-7 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83455-5-RR targets IFITM2 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IF/ICC, FC (Intra), ELISA Application Description |
经测试反应性 | human |
免疫原 | IFITM2 fusion protein Ag3451 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | interferon induced transmembrane protein 2 (1-8D) |
别名 | Interferon-induced transmembrane protein 2, IFITM 2, DSPA2c, 240318B6, 18D |
计算分子量 | 132 aa, 15 kDa |
观测分子量 | 15 kDa |
GenBank蛋白编号 | BC009696 |
基因名称 | IFITM2 |
Gene ID (NCBI) | 10581 |
RRID | AB_3671091 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purfication |
UNIPROT ID | Q01629 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
IFITM2, also named as 1-8D, belongs to the CD225 family. It is an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus, by inhibiting the early steps of replication. IFITM2 induces cell cycle arrest and mediates apoptosis by caspase activation and in a p53-independent manner. It is overexpressed in colon carcinoma. IFITM2 is a novel pro-apoptotic gene that will provide new insights into the regulated cellular pathways to death. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also, they serve as important components of the innate immune system to restrict HIV-1 infection.
实验方案
Product Specific Protocols | |
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WB protocol for IFITM2 antibody 83455-5-RR | Download protocol |
IF protocol for IFITM2 antibody 83455-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |